MTHFS Recombinant Protein

Source : Escherichia Coli. MTHFS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-203a.a.) and having a molecular mass of 25.4 kDa. MTHFS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. MTHFS is a cytosolic protein which takes part in the formate metabolic process. MTHFS along with a magnesium cofactor catalyzes the ATP-dependent reaction which reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate (MTHF) . MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. In addition, MTHF is a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD) .

Product Specifications

Product Name Alternative

5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) ||HsT19268||Methenyl-THF synthetase||FLJ30410||EC 6.3.3.2.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

The MTHFS protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 5mM DTT and 30% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

bs-8035R-Cy5.5 100 µL

GABARAPL1 Polyclonal Antibody, Cy5.5 Conjugated

Ask

View Details

NBP1-83678-25ul 25 µL

Rabbit Polyclonal ARSI Antibody

Ask

View Details

bs-9749R-PerCP 100 µL

ANKRD32 Polyclonal Antibody, PerCP Conjugated

Ask

View Details