MSRB Recombinant Protein

Source : Escherichia Coli. MSRB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids (1-137 a.a.) and having a molecular mass of 17.6kDa.MSRB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Methionine sulfoxide reductase B (MsrB) from Escherichia coli is a member of the msrB Met sulfoxide reductase family. The E.coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. msrB possess a metal binding site composed of 2 CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes.

Product Specifications

Product Name Alternative

Peptide methionine sulfoxide reductase MsrB||Peptide-methionine (R) -S-oxide reductase||msrB||yeaA||b1778||JW1767.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

MSRB protein solution (1mg/mL) 20mM Tris-HCl buffer (pH8.0), 20% glycerol 0.1M NaCl and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING.