MSRA Recombinant Protein

Source : Escherichia Coli. MSRA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (24-235) and having a molecular mass of 26.2kDa.The MSRA is fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. Methionine sulfoxide reductase A (MSRA) is a member of the MsrA Met sulfoxide reductase family. The MSRA enzyme has a vital function as a repair enzyme for proteins which have been inactivated by oxidation. MSRA catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. The three substrates of the MSRA enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, while its 2 products are peptide-L-methionine (R) -S-oxide and thioredoxin. The MSRA protein is ubiquitous and extremely conserved. Human and animal studies have shown the ultimate levels of expression in kidney and nervous tissue.

Product Specifications

Product Name Alternative

Mitochondrial peptide methionine sulfoxide reductase||Peptide-methionine (S) -S-oxide reductase||Peptide Met (O) reductase||Protein-methionine-S-oxide reductase||PMSR||MSRA.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

MSRA protein solution (0.5mg/mL) is supplied in 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT and 50mM NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGA ERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMR QGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNG YCGLGGTGVS CPVGIKK.