MMP 1 HEK Recombinant Protein

Source : HEK293 cells. MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-1 is purified by proprietary chromatographic techniques. MMP-1 (interstitial collagenase) can break down a wide range of substrates including types I, II, III, VII, VIII, and X collagens as well as L-Selectin, pro-TNF, IL-1, IGFBP-3, IGFBP-5, casein, gelatin, ?1 antitrypsin, myelin basic protein, pro-MMP2 and pro-MMP9. A significant function of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling. MMP-1 is expressed in fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 can be divided into a number of distinct domains: a prodomain which is cleaved on activation, a catalytic domain containing the zinc binding site and a short hinge region with a carboxyl terminal domain. MMP1 is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Product Specifications

Product Name Alternative

Interstitial collagenase||Fibroblast collagenase||Matrix metalloproteinase-1||MMP-1||MMP1||CLG||CLGN.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

The MMP-1 is supplied as a 0.2μm filtered solution in MES, NaCl, Glycerol and Brij35.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.

Applications Notes

The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pmoles/min/μg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA) .Activation Protocol:1. Dilute MMP1 to 50μg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours.