KDSR Recombinant Protein

Source : Escherichia Coli. KDSR Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 266 amino acids (26-270 a.a.) and having a molecular mass of 29kDa. The KDSR is purified by proprietary chromatographic techniques. 3-ketodihydrosphingosine reductase (KDSR) is a 332 amino acid multi-pass membrane protein which localizes to the ER and is a member of the short-chain dehydrogenases/reductases (SDR) family. KDSR is a secreted protein that is weakly expressed in hematopoietic tissue. Furthermore, KDSR catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS) . The putative active site residues of KDSR are found on the cytosolic side of the endoplasmic reticulum membrane. Chromosomal rearrangement in the KDSR gene is a cause of follicular lymphoma, aka type II chronic lymphatic leukemia.

Product Specifications

Product Name Alternative

3-ketodihydrosphingosine reductase||KDS reductase||3-dehydrosphinganine reductase||Follicular variant translocation protein 1||FVT-1||KDSR||FVT1||DHSR||SDR35C1||FLJ36555||FLJ92680.

Purification

KDSR was found to be greater than 90.0% as determined by SDS-PAGE.

Components

The KDSR solution (0.5 mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, 0.1M NaCl and 0.1mM PMSF.

Storage Conditions

KDSR should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MKPLALPGAH VVVTGGSSGI GKCIAIECYK QGAFITLVAR NEDKLLQAKK EIEMHSINDK QVVLCISVDV SQDYNQVENV IKQAQEKLGP VDMLVNCAGM AVSGKFEDLE VSTFERLMSI NYLGSVYPSR AVITTMKERR VGRIVFVSSQ AGQLGLFGFT AYSASKFAIR GLAEALQMEV KPYNVYITVA YPPDTDTPGF AEENRTKPLE TRLISETTSV CKPEQVAKQI VKDAIQGNFN SSLGSD.