What is the catalog number for GCLM Recombinant Protein?

The catalog number for GCLM Recombinant Protein is 32-2354-20.

What sizes are available for GCLM Recombinant Protein?

GCLM Recombinant Protein is available in the following size(s): 20 µg.

Is GCLM Recombinant Protein in stock?

GCLM Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

GCLM Recombinant Protein

Source : Escherichia Coli. GCLM Human Recombinant produced in E. coli is a single polypeptide chain containing 298 amino acids (1-274) and having a molecular mass of 33.3kDa.GCLM is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Glutamate-cysteine ligase (GCLM) is the first rate limiting enzyme of glutathione synthesis. The GCLM enzyme is comprised of 2 subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency is associated with some forms of hemolytic anemia.

Product Specifications

Product Name Alternative

Glutamate--cysteine ligase regulatory subunit||GCS light chain||Gamma-ECS regulatory subunit||Gamma-glutamylcysteine synthetase regulatory subunit||Glutamate--cysteine ligase modifier subunit||GCLM||GLCLR.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The GCLM solution (1mg/mL) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.15M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSHMGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS.