GATC Recombinant Protein

Source : Escherichia Coli. GATC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (1-136 a.a) and having a molecular mass of 17.5kDa (Molecular size on SDS-PAGE will appear higher) .GATC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Glutamyl-TRNA Amidotransferase, Subunit C also known as GATC allows the formation of properly charged Gln-tRNA (Gln) through the transamidation of misacylated Glu-tRNA (Gln) in the mitochondria. The reaction occurs in the attendance of glutamine and ATP through an activated gamma-phospho-Glu-tRNA (Gln) . In addition, GATC is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits.

Product Specifications

Product Name Alternative

Glutamyl-TRNA (Gln) Amidotransferase Subunit C||Glu-AdT Subunit C||15E1.2||Glutamyl-TRNA (Gln) Amidotransferase Subunit C Homolog (Bacterial) ||Glutamyl-TRNA (Gln) Amidotransferase Subunit C Mitochondrial||Glutamyl-TRNA (Gln) Amidotransferase Subunit C Ho

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

GATC protein solution (0.25mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS