BLVRA Recombinant Protein

Source : Escherichia Coli. BLVRA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids (3-296 a.a. and Methionine at N-terminus) and having a molecular mass of 33.3kDa (molecular weight on SDS-PAGE will shift up) .The BLVRA is purified by proprietary chromatographic techniques. Biliverdin reductase A (BLVRA) is a member of the gfo/idh/mocA family. BLVRA is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. BLVRA reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the simultaneous oxidation of a NADH or NADPH cofactor (Bilirubin + NAD (P) + = biliverdin + NAD (P) H) .BLVRA is a regulator for induction of activating transcription factor-2 and heme oxygenase-1. Furthermore, BLVRA enhances the role of HO-1 in cytoprotection and provides cytoprotection independent of heme degradation. In addition, Bilirubin while acting as a cytoprotective antioxidant is itself oxidized to biliverdin and subsequently recycled by biliverdin reductase back to bilirubin.

Product Specifications

Product Name Alternative

Biliverdin reductase A||BVR A||Biliverdin-IX alpha-reductase||BLVRA||BLVR||BVR||BVRA.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The BLVRA solution contains 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFGELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK.