BDH2 Recombinant Protein

Source : Escherichia Coli. BDH2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-245a.a.) and having a molecular mass of 28.8kDa.BDH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. BDH2 is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH2 protein has a significant part in the peripheral utilization of 3-hydroxybutyrate. BDH2 can convert high levels of circulating 3-hydroxybutyrate into acetoacetate due to cytoplasmic localization in high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters.

Product Specifications

Product Name Alternative

3-hydroxybutyrate dehydrogenase type 2||FLJ13261||PRO20933||SDR15C1||UCPA-OR||UNQ6308||dehydrogenase/reductase (SDR family) member 6||Oxidoreductase UCPA||DHRS6||R-beta-hydroxybutyrate dehydrogenase||EFA6R||EC 1.1.1.30.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

The BDH2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL