ATP5O Recombinant Protein

Source : Escherichia Coli. ATP5O Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 211 amino acids (24-213 a.a.) and having a molecular mass of 23.1kDa. The ATP5O is purified by proprietary chromatographic techniques. ATP synthase subunit O (ATP5O) localizes to the mitochondria and catalyzes ATP synthesis. ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. ATP5O seems to be part of the connector connecting these two components and may be involved in transmission of conformational changes or proton conductance.

Product Specifications

Product Name Alternative

ATP synthase subunit O mitochondrial||Oligomycin sensitivity conferral protein||OSCP||ATP5O||ATPO.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

The ATP5O solution (1 mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 40% glycerol and 0.2M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MFAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V.