MBRAK Recombinant Protein

Source : Escherichia Coli. CXCL14 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9.4kDa.The CXCL14 is purified by proprietary chromatographic techniques. CXCL14 is involved in immunoregulatory and inflammatory processes. BRAK protein is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. CXCL14 displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. CXCL14 is involved in the homeostasis of monocyte-derived macrophages.

Product Specifications

Product Name Alternative

C-X-C motif chemokine 14||B-cell and monocyte-activating chemokine||Chemokine BRAK||Kidney-expressed chemokine CXC||MIP-2G||Small-inducible cytokine B14||Cxcl14||Bmac||Kec||Ks1||Mip2g||Scyb14||BRAK||NJAC||AI414372||bolekine||MIP2gamma||1110031L23Rik

Purification

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

CXCL14 was lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH 7.4 and 500mM NaCl.

Storage Conditions

Lyophilized CXCL14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL14 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized CXCL14 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 of CXCL14 as determined by its ability to chemoattract activated monocytes using a concentration range of 1.0-10.0 ng/ml.