MTPO Recombinant Protein

Source : Escherichia Coli. Thrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton. The TPO is purified by proprietary chromatographic techniques. Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidneyt hat regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.

Product Specifications

Product Name Alternative

Megakaryocyte colony-stimulating factor||Myeloproliferative leukemia virus oncogene ligand||C-mpl ligand||ML||Megakaryocyte growth and development factor||MGDF||TPO||MKCSF||MPLLG||MGC163194||THPO.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

Lyophilized from a concentrated (1mg/mL) solution in water containing no additives.

Storage Conditions

Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO Mouse should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Thrombopoietin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. ED50 range is less than 0.8ng/ml as determined by the dose dependent stimulation of Mo7e cells.

Amino Acids

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF.