TNFRSF10B Recombinant Protein

Source : Escherichia Coli. TNFRSF10B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 132 amino acids and having a molecular mass of 14.8kDa.The TNFRSF10B is purified by proprietary chromatographic techniques. TRAIL Receptor-1 (DR4) and TRAIL Receptor-2 (DR5) are members of the TNFR superfamily of transmembrane proteins and contain a cytoplasmic 'death domain', which is capable of activating the cell's apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

Product Specifications

Product Name Alternative

Tumor necrosis factor receptor superfamily member 10B||Death receptor 5||TNF-related apoptosis-inducing ligand receptor 2||TRAIL receptor 2||TRAIL-R2||CD262||TNFRSF10B||DR5||KILLER||TRAILR2||TRICK2||ZTNFR9||TRICKB||TRICK2A||TRICK2B||KILLER/DR5.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Storage Conditions

Lyophilized TNFRSF10B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF10B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized TNFRSF10B in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The TNFRSF10B reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10ng/mL) but in the presence of TNFRSF10B, TRAIL-induced TNF is suppressed.