TNF b His Recombinant Protein

Source : Escherichia Coli. TNF-b Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids (35-205 a.a.) and having a molecular mass of 20.9kDa. TNF-b is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. TNF-b is a potent multifunctional cytokine produced mainly by activated T and B lymphocytes. The protein has been shown to have a specific potent cytotoxic activity on tumor cells and a variety of other target cells as well as being a mediator of inflammation and immune function.

Product Specifications

Product Name Alternative

Lymphotoxin-alpha||LT-alpha||TNF-beta||Tumor necrosis factor ligand superfamily member 1||LTA||LT||TNFB||TNFSF1.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

TNF-b protein solution (0.25mg/mL) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Applications Notes

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is

Amino Acids

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.