SCF Sf9 Recombinant Protein

Source : Sf9, Insect Cells. Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques. Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit) . SCF is also known as 'steel factor' or 'c-kit ligand'. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid) .

Product Specifications

Product Name Alternative

Kit ligand Precursor||C-kit ligand||SCF||Mast cell growth factor||MGF||SF||KL-1||Kitl||DKFZp686F2250.

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The protein is supplied in 1X PBS, pH 7.4.

Storage Conditions

Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.