MsRANKL Recombinant Protein

Source : Escherichia Coli. sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9kDa.CD254 is purified by proprietary chromatographic techniques. RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Product Specifications

Product Name Alternative

Soluble Receptor Activator of NFkB Ligand||TNFSF11||TRANCE||TNF-related activation-induced cytokine||OPGL||ODF||Osteoclast differentiation factor||Tumor necrosis factor ligand superfamily member 11||Receptor activator of nuclear factor kappa B ligand

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

The protein (1mg/mL) was lyophilized with 10mM Na2PO4, pH 7.5 & 50mM NaCl.

Storage Conditions

Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in â€œCorning® Osteo Assay Surface 24 Well Plates with Transwell® Permeable Supports- A Useful Tool for Co-Culture Studiesâ€ by Rebecca M. Wood and Mark Rothenber, corresponding to a specific activity of 20,000Units/mg.

Amino Acids

PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.