PLGF 2 Recombinant Protein

Source : Insect Cells. Placenta Growth Factor-2 Human Recombinant produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 152 amino acids and having a total molecular mass of 44 kDa. The PLGF-2 is purified by proprietary chromatographic techniques. PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.PLGF-2 binds neuropilin-1 and 2 in a heparin-dependent manner.

Product Specifications

Product Name Alternative

PIGF||PGF||PlGF-2||PLGF-2.

Purification

Greater than 80.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

Lyophilized from a concentrated (1mg/mL) solution containing BSA.

Storage Conditions

Lyophilized Placenta Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF2 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Placenta Growth Factor 2 in sterile 20mM acetic acid not less than 100μg/ml, which can then be further diluted to other aqueous solutions. PlGF-2 human Recombinant can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.3¢â‚¬â€œ10ng/ml.