NRN1 Recombinant Protein

Source : Escherichia Coli. Recombinant Human NRN1 produced in E.coli cells is a non-glycosylated, homodimeric protein containing 2x88 amino acid chains and having a molecular mass of 19.4kDa. The NRN1 is purified by proprietary chromatographic techniques. Neuritin (NRN1) is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3 and other neural stimulators. NRN1 is expressed principally in postmitotic-differentiating neurons of the developing nervous system and in neuronal structures related to synaptic plasticity in the adult nervous system. NRN1 functions as a molecular mediator of neurite outgrowth, neuronal survival, and synaptic maturation.

Product Specifications

Product Name Alternative

Neuritin 1||NRN1||NRN||dJ380B8.2||Neuritin.

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The NRN1 was lyophilized from a 0.2μm filtered concentrated solution in 1xPBS, pH 7.4.

Storage Conditions

Lyophilized NRN1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NRN1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized NRN1 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 25ng/ml, corresponding to a specific activity of > 4.0 ƒâ€” 104 IU/mg.