NRGN Recombinant Protein

Source : Escherichia Coli. NRGN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 101 amino acids (1-78 a.a.) and having a molecular mass of 10.0kDa (molecular size on SDS-PAGE will appear higher) . NRGN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Neurogranin (NRGN) is a calmodulin-binding protein which is expressed solely in the brain, mainly in dendritic spines. NRGN is also taking part in the protein kinase C signaling pathway by being a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. NRGN binds to calmodulin in the absence of calcium. NRGN protein's phosphorylation by protein kinase C lowers its binding ability.

Product Specifications

Product Name Alternative

Hng||RC3||Neurogranin||Ng||NRGN.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

NRGN protein solution (0.5mg/mL) contains 20mM Tris-HCl buffer (pH7.0), 30% glycerol 0.1mM PMSF and 1mM EDTA.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.