MANF Recombinant Protein

Source : Escherichia Coli. MANF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18.1 kDa. The MANF is purified by proprietary chromatographic techniques. MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

Product Specifications

Product Name Alternative

Mesencephalic astrocyte-derived neurotrophic factor||Protein ARMET||ARP||arginine-rich mutated in early stage tumors||Arginine-rich protein.

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

Filtered (0.2μm) and lyophilized from a concentrated (1mg/mL) solution in 1xPBS, pH7.4.

Storage Conditions

Lyophilized MANF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MANF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized MANF in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 was determined by its ability to stimulate the proliferation of rat C6 cells is typically 15-25 μg/ml.

Amino Acids

LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

EK771079 96 Well

Goat adrenocorticotropic hormone (ACTH) Elisa Kit

Ask

View Details

MBS1353733-01 0.02 mg (E-Coli)

Recombinant Oryza sativa subsp. japonica Thioredoxin H4-2 (Os05g0169000, LOC_Os05g07690)

Ask

View Details

MBS1353733-02 0.1 mg (E-Coli)

Recombinant Oryza sativa subsp. japonica Thioredoxin H4-2 (Os05g0169000, LOC_Os05g07690)

Ask

View Details

MBS1353733-03 0.02 mg (Yeast)

Recombinant Oryza sativa subsp. japonica Thioredoxin H4-2 (Os05g0169000, LOC_Os05g07690)

Ask

View Details

MBS1353733-04 0.1 mg (Yeast)

Recombinant Oryza sativa subsp. japonica Thioredoxin H4-2 (Os05g0169000, LOC_Os05g07690)

Ask

View Details

MBS1353733-05 0.02 mg (Baculovirus)

Recombinant Oryza sativa subsp. japonica Thioredoxin H4-2 (Os05g0169000, LOC_Os05g07690)

Ask

View Details