IL1F10 Recombinant Protein

Source : Escherichia Coli. IL1F10 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 152 amino acids and having a molecular mass of 17kDa.The IL1F10 is purified by proprietary chromatographic techniques. Human interleukin family 1, member 10 (IL1F10) belongs to the interleukin 1 cytokine family. IL1F10 is expressed in the fetal skin, spleen and tonsil, generally in the basal epithelia of skin and in proliferating B-cells of the tonsil. IL1F10 binds soluble IL1 receptor type 1 and may be implicated in the regulation of adapted and innate immune responses.

Product Specifications

Product Name Alternative

Interleukin-1 family member 10||IL-1F10||FIL1 theta||Interleukin-1 HY2||IL-1HY2||Interleukin-1 theta||IL-1 theta||IL1F10||FIL1T||IL1HY2||FKSG75||MGC119831||MGC119832||MGC119833||FIL1-theta.

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

IL1F10 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.

Storage Conditions

Lyophilized IL1F10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1F10 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to quick spin followed by reconstitution of IL1F10 in PBS to a concentration no less than 100 μg/ml, which can then be further diluted to other aqueous solutions. As measured by its binding ability in a functional ELISA, immobilized IL1F10 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15- 5 μg/ml.