MIL36B Recombinant Protein

Source : Escherichia Coli. IL36B Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 183 amino acids and having a molecular mass of 21.0kDa.The IL36B is purified by proprietary chromatographic techniques. Mouse IL-36b belongs to the IL-1 family that includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36ra (IL1F5), IL-36b (IL1F8), IL-36g (IL1F9), IL-37 (IL1F7) and IL-38 (IL-1F10) . The IL-1 family members display a 12 b-strand, b-trefoil configuration, and are thought to have ascended from a mutual ancestral gene. Mouse IL-36b/IL-1F8 is a 183 amino acid protein which holds no signal sequence, no prosegment and no potential N-linked glycosylation site (s) . Mouse IL-36b like its human homologue is actively secreted. Mouse IL-36/IL-1F8 shares 61-74% aa homology with human IL-36/IL-1F8 isoform 2 and rat IL-1F8.

Product Specifications

Product Name Alternative

Interleukin 36 beta||interleukin 1 family member 8 (eta) ||Interleukin-1 homolog 2||IL1F8 (Canonical product IL-1F8a) ||IL-1F8 (FIL1-eta) ||Interleukin-1 Superfamily e||IL1H2||MGC126880||MGC126882.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

Lyophilized from a 0.2μm filtered concentrated solution in 1xPBS, pH 7.4.

Storage Conditions

Lyophilized IL36B Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36B should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized IL36B in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Measured by its binding ability in a functional ELISA to bind recombinant mouse IL-1 Rrp2.

Amino Acids

MMAFPPQSCV HVLPPKSIQM WEPNHNTMHG SSQSPRNYRV HDSQQMVWVL TGNTLTAVPA SNNVKPVILS LIACRDTEFQ DVKKGNLVFL GIKNRNLCFC CVEMEGKPTL QLKEVDIMNL YKERKAQKAF LFYHGIEGST SVFQSVLYPG WFIATSSIER QTIILTHQRG KLVNTNFYIE SEK