IL36RN Recombinant Protein

Source : Escherichia Coli. IL1F5 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 155 amino acids and having a molecular mass of 17kDa.The IL1F5 is purified by proprietary chromatographic techniques. Human interleukin family 1, member 5 (IL-1F5 / FIL1-delta) belongs to the interleukin 1 cytokine family. IL1F5 is expressed by a variety of cells including monocytes, Bcells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells. IL1F5 is an antagonist of IL1F9; however IL1F5 activity related to receptor binding remains unclear. Human and mouse IL1F5 share 90% amino acid sequence identity.

Product Specifications

Product Name Alternative

Interleukin-36 receptor antagonist protein||FIL1 delta||IL-1-related protein 3||IL-1RP3||Interleukin-1 HY1||IL-1HY1||Interleukin-1 delta||IL-1 delta||Interleukin-1 family member 5||IL-1F5||Interleukin-1 receptor antagonist homolog 1||IL-1ra homolog 1

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

IL1F5 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.

Storage Conditions

Lyophilized IL1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1F5 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to quick spin followed by reconstitution of IL1F5 in PBS to a concentration no less than 100 μg/ml, which can then be further diluted to other aqueous solutions. As measured by its binding ability in a functional ELISA, immobilized IL1F5 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15- 5 μg/ml.