IGF2 Recombinant Protein

Source : Escherichia Coli. Insulin-Like Growth Factor-II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton. IGF-II is purified by proprietary chromatographic techniques. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Specifications

Product Name Alternative

Somatomedin-A||IGF2||INSIGF||pp9974||C11orf43||FLJ22066||FLJ44734.

Purification

Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The protein was lyophilized with no additives.

Storage Conditions

Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFII should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized IGF2 in sterile 18MΩ-cm H2O at a concentration of 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.