IFNB 1b Recombinant Protein

Source : Escherichia Coli. Interferon beta 1b Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. IFN beta-1b is purified by proprietary chromatographic techniques. Interferon-beta 1b has antiviral, antibacterial and anticancer activities.

Product Specifications

Product Name Alternative

Leukocyte interferon||B cell interferon||Type I interferon||IFNB1||IFB||IFF||IFNB||IFN-b 1b||MGC96956.

Purification

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

Lyophilized from 1mg/mL solution containing 50mg Human Albumin & 50mg dextrose.

Storage Conditions

Lyophilized Interferon-beta 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNB 1b should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18M?-cm H2O at a concentration of 0.25mg/ml, which can then be further diluted to other aqueous solutions. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32 x 106 IU/mg.