Flt3 Ligand His Recombinant Protein

Source : Escherichia Coli. Flt3-Ligand Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 165 amino acids (including his tag) and having a molecular mass of 18.9 kDa. Flt3-Ligand is purified by proprietary chromatographic techniques. FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.

Product Specifications

Product Name Alternative

Fms-related tyrosine kinase 3 ligand||FLK2||STK1||CD135||Stem Cell Tyrosine Kinase 1||FLT3LG||Flt3.

Purification

Greater than 97 % as determined by SDS-PAGE.

Components

The protein was lyophilized with PBS.

Storage Conditions

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The activity was measured in a cell proliferation assay. The EC50 for this effect is typically 0.2-1 ng/mL.