FAS Recombinant Protein

Source : Escherichia Coli. sFas Receptor Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.6kDa.The FAS is purified by proprietary chromatographic techniques. Fas and Fas Ligand (FasL) are members of the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas initiates apoptosis in Fas-bearing cells. The apoptosis mechanism involves the recruitment of pro-caspase 8 through an adaptor molecule named FADD followed by processing of the pro-enzyme to active forms. These active caspases subsequently cleave a variety of cellular substrates leading to the eventual cell death. sFasR is able to inhibit FasL-induced apoptosis by acting as a decoy receptor whicht serves as a sink for FasL. The full length Fas Receptor is a 319 a.a type I transmembrane protein, which contains a 157 a.a extracellular domain, a 17 a.a transmembrane domain, and 145 a.a cytoplasmic domain. The mature human Fas ECD shares 55%, 58%, a.a sequence identity with the mouse, rat, Fas, respectively.

Product Specifications

Product Name Alternative

Tumor necrosis factor receptor superfamily member 6||Apo-1 antigen||Apoptosis-mediating surface antigen FAS||FASLG receptor||CD95||FAS||APT1||FAS1||APO-1||FASTM||ALPS1A||TNFRSF6.

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

FAS protein was lyophilized from a 0.2μm filtered concentrated solution in 1xPBS, pH 7.4.

Storage Conditions

Lyophilized FAS although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FAS should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized FAS in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 μg/ml in the presence of 2ng/ml of hFasL.

Amino Acids

MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.