MEBI3 Recombinant Protein

Source : Escherichia Coli. EBI3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 22.9kDa. The Murine EBI3 is purified by proprietary chromatographic techniques. EBI3 has an induced expression in B lymphocytes in reaction to Epstein-Barr virus infection. EBI3 encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form iIL-27. EBI3 drives rapid clonal expansion of naive cd4 (+) t-cells. EBI3 strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4 (+) t-cells. EBI3 mediates its biologic effects through the cytokine receptor wsx-1/tccr.

Product Specifications

Product Name Alternative

IL-27B||IL27B||IL 27-B||EBI-3||Interleukin-27 beta||IL-27 subunit beta||Epstein-Barr virus-induced gene 3 protein homolog||EBI3.

Purification

Greater than 90% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

EBI3 was lyophilized from 10mM Sodium Citrate pH-3.

Storage Conditions

Lyophilized EBI3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EBI3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized EBI3 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Amino Acids

MALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP.