BD5 Recombinant Protein

Source : Escherichia Coli. BD5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 5.8 kDa. The Beta Defensin-5 is purified by proprietary chromatographic techniques. Defensins (alpha & beta) are cationic peptides with a wide spectrum of antimicrobial activity which include a significant arm of the innate immune system. There are 6 human beta-defensins, BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6 which are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, beta-defensins can act as chemoattractants towards immature dendritic cells and memory T cells. Beta-defensins contain a 6-cysteine motif which forms 3 intra-molecular disulfide bonds.

Product Specifications

Product Name Alternative

Beta-defensin 105||eta-defensin 5||BD-5||DEFB-5||Defensin||beta 105||DEFB105A||BD5||DEFB105||DEFB5||DEFB105B.

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Storage Conditions

Lyophilized Beta Defensin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Beta Defensin-5 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.