Anti-Tocilizumab Antibody (Clone: 6C10), mAb, Mouse

Tocilizumab also known as atlizumab, is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration. It has a high affinity to IL-6R and blocks the IL-6 signaling by preventing the formation of the IL-6/IL-6R complex. Tocilizumab is used for the trea„¢ent of rheumatoid arthritis (RA) and systemic juvenile idiopathic arthritis, a severe form of arthritis in children. Anti-Tocilizumab Antibody (6C10), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Tocilizumab.

Product Specifications

Product Name Alternative

Mouse monoclonal to Actemra

Host

Mouse

Immunogen

Tocilizumab

Target Antigen

Tocilizumab

Clonality

Monoclonal

Clone

6C10

Applications

ELISA

Purification

Protein A chromatography

Format

Purified

Components

Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

Reconstitute Lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration range is a recommended starting point for this product. ELISA detection: 0.01-0.1 μg/mL ELISA blocking: 1-30 μg/mL