Rabbit Monoclonal Antibody to His Tag (C-term) (Clone : 25B6E11)

The rabbit immune system generates antibody diversity and optimizes affinity. technology to generate high affinity and specific monoclonal rabbit antibodies. Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. This product is not recommended for N-terminal His-fusion protein detection.

Product Specifications

Host

Rabbit

Immunogen

6x His synthetic peptide coupled to KLH

Clonality

Monoclonal

Clone

25B6E11

Applications

IP, ELISA, ICC/IF, FACS, WB

Purification

Protein A chromatography

Format

Purified

Components

Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. Reconstitute with deionized water or equivalent to 0.5 mg/mL.

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.05-0.2 μg/mL Western Blot: 1-2 μg/mL Immunoprecipitation (IP) : 2 μg/mL Immunocytochemistry/Immunofluorescence (ICC/IF) : 1 μg/mL Flow Cytometry: 1 μg/mL