Mouse Monoclonal Antibody to Human PD-L1 (Clone : PDL1.D1)

Programmed cell death 1 ligand 1 is one of the two ligands of PD-1. PD-L1 expresses on macrophages, T cells, B cells, NK cells, DCs and some cancer cell surface. Binding of PD-1 with PD-L1 could result in down-regulation of the immune system by inhibiting the T-cell activation process. Thus, PD-L1 is an important immune checkpoint and popular target for therapeutic antibodies against many cancers. Anti-Human PD-L1 Antibody (PDL1.D1), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human PD-L1-Fc .

Product Specifications

Product Name Alternative

Programmed cell death protein 1, Protein PD-1, hPD-1, CD279

Gene Name

PDCD1

Gene ID

5133

UniProt

Q15116

Host

Mouse

Immunogen

Recombinant human PD-L1-Fc

Clonality

Monoclonal

Clone

PDL1.D1

Applications

ELISA

Purification

Protein A chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA detection: 0.01-0.1 μg/mL ELISA blocking: 10-15 μg/mL Flow cytometry: 5-7 μg/mL Blockade of Receptor-ligand Interaction in Flow cytometry: 5-7 μg/mL