Mouse Monoclonal Antibody to Human PD-1 (Clone : PD1.D3)

Programmed cell death protein 1 (PD-1), is cell surface receptor expressing on T cells and pro-B cells. Binding of its two ligands PD-L1 and PD-L2 could result in down-regulation of the immune system by inhibiting the T-cell activation process. Thus, PD-1 is an important immune checkpoint and popular target for therapeutic antibodies against many cancers. Anti-Human PD-1 Antibody (PD1.D3), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human PD-1-Fc .

Product Specifications

Product Name Alternative

Programmed cell death protein 1, Protein PD-1, hPD-1, CD279

Gene Name

PDCD1

Gene ID

5133

UniProt

Q15116

Host

Mouse

Immunogen

Recombinant human PD-1-Fc

Clonality

Monoclonal

Clone

PD1.D3

Applications

ELISA

Purification

Protein A chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA detection: 0.01-0.1 μg/mL ELISA blocking: 10-15 μg/mL Flow cytometry: 5-10 μg/mL Blockade of Receptor-ligand Interaction in Flow cytometry: 5 μg/mL