Rabbit Polyclonal Antibody to GFP
Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm) . GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants. Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Product Specifications
Host
Rabbit
Immunogen
Purified recombinant full-length GFP protein
Clonality
Polyclonal
Applications
ELISA, WB
Purification
Immunoaffinity chromatography
Format
Purified
Components
0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Storage Conditions
Applications Notes
ELISA: 0.05-0.2 ‚μg/mL.Western blot: 1-2 ‚μg/mL
Isotype
Rabbit IgG
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