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Rabbit Polyclonal Antibody to GFP

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm) . GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants. Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.

Product Specifications

Host

Rabbit

Immunogen

Purified recombinant full-length GFP protein

Clonality

Polyclonal

Applications

ELISA, WB

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.05-0.2 ‚μg/mL.Western blot: 1-2 ‚μg/mL

Isotype

Rabbit IgG

Frequently Asked Questions

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