HRP conjugated Rabbit Polyclonal Antibody to E-tag

Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags.

Product Specifications

Host

Rabbit

Reactivity

Human

Immunogen

E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH

Clonality

Polyclonal

Applications

WB, ELISA

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.05-0.2 ‚μg/mLWestern blot:1-2 ‚μg/mL Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.