Rabbit Polyclonal Antibody to RFP-tag

Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.

Product Specifications

Host

Rabbit

Immunogen

RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH

Clonality

Polyclonal

Applications

ELISA

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.05-0.2 μg/mL Western blot: 1-2 μg/mL . Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.