Rabbit Polyclonal Antibody to Avi-tag

Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.

Product Specifications

Host

Rabbit

Immunogen

Avi tag peptide conjugated to KLH

Clonality

Polyclonal

Applications

ELISA, WB

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.05-0.2 ‚μg/mLWestern blot: 1-2 ‚μg/mL Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.