Rabbit Polyclonal Antibody to Avi-tag
Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
Product Specifications
Host
Rabbit
Immunogen
Avi tag peptide conjugated to KLH
Clonality
Polyclonal
Applications
ELISA, WB
Purification
Immunoaffinity chromatography
Format
Purified
Components
0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Storage Conditions
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Applications Notes
ELISA: 0.05-0.2 ‚μg/mLWestern blot: 1-2 ‚μg/mL Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Isotype
Rabbit IgG
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