Rabbit Polyclonal Antibody to His-tag
His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.
Product Specifications
Host
Rabbit
Immunogen
His epitope tag peptide HHHHHH conjugated to KLH
Clonality
Polyclonal
Applications
ELISA
Purification
Immunoaffinity chromatography
Format
Purified
Components
0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Storage Conditions
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Applications Notes
ELISA: 0.05-0.2 μg/mL
Isotype
Rabbit IgG
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