Rabbit Polyclonal Antibody to His-tag

His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.

Product Specifications

Host

Rabbit

Immunogen

His epitope tag peptide HHHHHH conjugated to KLH

Clonality

Polyclonal

Applications

ELISA

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.