Rabbit Polyclonal Antibody to c-Myc-tag

The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Product Specifications

Host

Rabbit

Reactivity

Human

Immunogen

C-Myc epitope tag peptide EQKLISEEDL conjugated to KLH

Clonality

Polyclonal

Applications

ELISA

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.05-0.2 μg/mL Western blot: 1-2 μg/mL Immunoprecipitation (IP) : 2-5 μg/mL Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.