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Rabbit Polyclonal Antibody to DYKDDDDK-tag

The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.

Product Specifications

Host

Rabbit

Immunogen

A synthetic peptide DYKDDDDK conjugated to KLH

Clonality

Polyclonal

Applications

ELISA

Purification

Immunoaffinity chromatography

Format

Purified

Components

0.5 mg/mL, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Storage Conditions

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications Notes

ELISA: 0.01-0.05 μg/mL Western blot: 1-2 μg/mL Immunofluorescence:10-20 μg/mL

Isotype

Rabbit IgG

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