Anti-Fibronectin Monoclonal Antibody (Clone: FN-15)

Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.

Product Specifications

Product Name Alternative

Fibronectin; FN; Anastellin; Fn1

Gene Name

Fn1

UniProt

P04937

Host

Mouse

Reactivity

Human

Immunogen

Fibronectin from human plasma.

Target Antigen

Fibronectin

Clonality

Monoclonal

Clone

FN-15

Applications

ICC, WB

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 1-2μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 1-2μg/ml; Immunohistochemistry (Frozen Section) : 1-2μg/ml; Immunocytochemistry : 1μg/ml