Anti-PCNA Monoclonal Antibody (Clone: PC 10)

Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Product Specifications

Product Name Alternative

ProlifeRating cell nuclear antigen; PCNA; Cyclin; Pcna

Gene Name

Pcna

Gene ID

25737

UniProt

P04961

Host

Mouse

Reactivity

Human

Immunogen

Protein A fusion protein.

Target Antigen

Proliferating cell nuclear antigen

Clonality

Monoclonal

Clone

PC 10

Applications

WB, IHC-P, IHC-F, ICC

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 2μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 0.4-1μg/ml; Immunohistochemistry (Frozen Section) : 0.4-1μg/ml; Immunocytochemistry : 1μg/ml