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Anti-p34cdc2 Monoclonal Antibody (Clone: A17)

P34 (cdc2), also known as cell division cycle (CDC2), or cyclin-dependent kinase 1 (CDK1) . CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase 1; CDK1; 2.7.11.22; 2.7.11.23; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; Cdk1; Cdc2, Cdc2a, Cdkn1

Gene Name

Cdk1

Gene ID

54237

UniProt

P39951

Host

Mouse

Reactivity

Human

Immunogen

C-terminal two-thirds of Xenopus p34cdc2 expressed in E.coli.

Target Antigen

Cyclin-dependent kinase 1

Clonality

Monoclonal

Clone

A17

Applications

WB, IHC-P, IHC-F

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 0.5-1μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 1-2μg/ml; Immunohistochemistry (Frozen Section) : 1-2μg/ml

Isotype

Mouse IgG2a

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