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Anti-alpha-Smooth Muscle Actin Monoclonal Antibody (Clone: 1A4)

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Product Specifications

Product Name Alternative

Actin, aortic smooth muscle; Alpha-actin-2; Acta2; Actsa, Actvs

Gene Name

Acta2

Gene ID

81633

UniProt

P62738

Host

Mouse

Reactivity

Human

Immunogen

N-terminal synthetic decapeptide of alpha-smooth muscle actin.

Target Antigen

Actin, aortic smooth muscle

Clonality

Monoclonal

Clone

1A4

Applications

WB, IHC-P, IHC-F

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 2μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 0.1-0.5μg/ml; Immunohistochemistry (Frozen Section) : 0.1-0.5μg/ml

Isotype

Mouse IgG2a

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