Anti-Vinculin Monoclonal Antibody (Clone: hVIN-1)
Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%) . Vinculinis mapped to 10q22.1-q23.
Product Specifications
Product Name Alternative
Vinculin ; Metavinculin ; Vcl
Gene Name
Vcl
Gene ID
305679
UniProt
P85972
Host
Mouse
Reactivity
Human
Immunogen
Human vinculin, purified from uterus.
Target Antigen
Vinculin
Clonality
Monoclonal
Clone
HVIN-1
Applications
WB, IHC-F
Purification
Ascites
Components
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.
Storage Conditions
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Applications Notes
Western blot : 1-2μg/ml; Immunohistochemistry (Frozen Section) : 2-4μg/ml
Isotype
Mouse IgG1
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