Anti-HSP25 Monoclonal Antibody (Clone: IAP-9)

The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.

Product Specifications

Product Name Alternative

Heat shock protein beta-1; HspB1; Heat shock 27 kDa protein; HSP 27; Hspb1; Hsp27

Gene Name

HSPB1

Gene ID

3315

UniProt

P42930

Host

Mouse

Reactivity

Human

Immunogen

Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

Target Antigen

Heat shock protein beta-1

Clonality

Monoclonal

Clone

IAP-9

Applications

ICC, WB

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 0.5-2μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 1-2μg/ml; Immunohistochemistry (Frozen Section) : 1-2μg/ml; Immunocytochemistry : 1μg/ml