Anti-Cytokeratin Peptide 18 Monoclonal Antibody (Clone: CY-90)

Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Product Specifications

Product Name Alternative

CK 18, CK18, CYK18, Cytokeratin endo B, K18

Gene Name

KRT18

Gene ID

3875

UniProt

P05783

Host

Mouse

Reactivity

Human

Immunogen

The Human epidermal carcinoma A-431 and MCF-7 Human breast cancer cell lines.

Target Antigen

Keratin, type I cytoskeletal 18

Clonality

Monoclonal

Clone

CY-90

Applications

WB, IHC-P, IHC-F

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.