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Anti-Cytokeratin Peptide 7 Monoclonal Antibody (Clone: LDS-68)

KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

Product Specifications

Product Name Alternative

K7, CK7, SCL, K2C7, MGC3625

Gene Name

KRT7

Gene ID

3855

UniProt

P08729

Host

Mouse

Reactivity

Human

Immunogen

Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Target Antigen

Keratin, type II cytoskeletal 7

Clonality

Monoclonal

Clone

LDS-68

Applications

WB, IHC-P, IHC-F

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 1-2μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 2-4μg/ml; Immunohistochemistry (Frozen Section) : 2-4μg/ml

Isotype

Mouse IgG1

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