Anti-Bcl-2 Monoclonal Antibody (Clone: Bcl-2-100)

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Product Specifications

Product Name Alternative

Apoptosis regulator Bcl-2; Bcl2; Bcl-2

Gene Name

Bcl2

Gene ID

24224

UniProt

P49950

Host

Mouse

Reactivity

Human

Immunogen

Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Target Antigen

Apoptosis regulator Bcl-2

Clonality

Monoclonal

Clone

Bcl-2-100

Applications

WB, IHC-P, IHC-F, ICC

Purification

Ascites

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/mL.

Storage Conditions

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Applications Notes

Western blot : 1-2μg/ml; Immunohistochemistry (Paraffin-embedded Section) : 0.4-1μg/ml; Immunohistochemistry (Frozen Section) : 0.4-1μg/ml; Immunocytochemistry : 1μg/ml